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csy4 expression cassette  (GenScript corporation)

 
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    GenScript corporation csy4 expression cassette
    One‐step generation of multiple precise genome edits via the <t>Csy4‐mediated</t> processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.
    Csy4 Expression Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/csy4 expression cassette/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    csy4 expression cassette - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression"

    Article Title: Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression

    Journal: Advanced Science

    doi: 10.1002/advs.202417790

    One‐step generation of multiple precise genome edits via the Csy4‐mediated processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.
    Figure Legend Snippet: One‐step generation of multiple precise genome edits via the Csy4‐mediated processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.

    Techniques Used: Mutagenesis, Plasmid Preparation



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    csy4 expression cassette  (GenScript corporation)
    90
    GenScript corporation csy4 expression cassette
    One‐step generation of multiple precise genome edits via the <t>Csy4‐mediated</t> processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.
    Csy4 Expression Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/csy4 expression cassette/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    csy4 expression cassette - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

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    One‐step generation of multiple precise genome edits via the Csy4‐mediated processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.

    Journal: Advanced Science

    Article Title: Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression

    doi: 10.1002/advs.202417790

    Figure Lengend Snippet: One‐step generation of multiple precise genome edits via the Csy4‐mediated processing system. A) Structure of the vectors of three‐pegRNAs containing cPE2 and epiPE2 and statistical analysis of the efficiencies of individual pegRNAs. B) Summary of mutation frequencies at three target sites by the three‐pegRNA epiPE2‐Csy4 system in HEK293T cells. C) Schematic representation of the genotyping results for all 48 cell colonies categorized as biallelic mutation, monoallelic mutation and wild type. D) Structure of the vector used to express pegRNAs 1 to 10 via the U6 promoter and efficiency analysis. E) Structure of the vector used to express pegRNAs 1 to 10 via the EF1α promoter and efficiency analysis.

    Article Snippet: To produce the epiPE2‐Csy4 vector, the Csy4 expression cassette was synthesized by blocking the AarI site (Genscript) and fused to epiPE2.

    Techniques: Mutagenesis, Plasmid Preparation